On-chip analysis of glycolysis and mitochondrial respiration in human induced pluripotent stem cells.

ABSTRACT: Recent advances in microfluidic engineering allow the creation of microenvironments in which human cells can be cultured under (patho-)physiological conditions with greater reality than standard plastic tissue culture plates. Microfluidic devices, also called Organs-on-Chip (OoC), allow complex engineering of the cellular compartment, yielding designs in which microfluidic flow can be precisely controlled. However, it is important that cellular physiology is not only controlled but can also be monitored in these devices. Here, we integrated oxygen and pH sensors into microfluidics, allowing close monitoring of the extracellular flux from the cells, enabling constant assessment of features such as glycolysis and mitochondrial oxidative phosphorylation in situ. Using human-induced plu

SUBMITTER: Fuchs S 

PROVIDER: S-EPMC9647220 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature