Constructing metagenome-assembled genomes for almost all components in a real bacterial consortium for binning benchmarking.

ABSTRACT:

Background

So far, a lot of binning approaches have been intensively developed for untangling metagenome-assembled genomes (MAGs) and evaluated by two main strategies. The strategy by comparison to known genomes prevails over the other strategy by using single-copy genes. However, there is still no dataset with all known genomes for a real (not simulated) bacterial consortium yet.

Results

Here, we continue investigating the real bacterial consortium F1RT enriched and sequenced by us previously, considering the high possibility to unearth all MAGs, due to its low complexity. The improved F1RT metagenome reassembled by metaSPAdes here utilizes about 98.62% of reads, and a series of analyses for the remaining reads suggests that the possibility of containing other low-abundance

SUBMITTER: Wu Z 

PROVIDER: S-EPMC9647946 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature