Characterization of Two Novel EF-Hand Proteins Identifies a Clade of Putative Ca²⁺-Binding Protein Specific to the Ambulacraria.

ABSTRACT: In recent years, transcriptomic databases have become one of the main sources for protein discovery. In our studies of nervous system and digestive tract regeneration in echinoderms, we have identified several transcripts that have attracted our attention. One of these molecules corresponds to a previously unidentified transcript (Orpin) from the sea cucumber Holothuria glaberrima that appeared to be upregulated during intestinal regeneration. We have now identified a second highly similar sequence and analyzed the predicted proteins using bioinformatics tools. Both sequences have EF-hand motifs characteristic of calcium-binding proteins (CaBPs) and N-terminal signal peptides. Sequence comparison analyses such as multiple sequence alignments and phylogenetic analyses only sho

SUBMITTER: Soto-Acaba A 

PROVIDER: S-EPMC9648499 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature