Manipulation of Ion Types via Gas-Phase Ion/Ion Chemistry for the Structural Characterization of the Glycan Moiety on Gangliosides.

ABSTRACT: Gangliosides are the most abundant glycolipid among eukaryotic cell membranes and consist of a glycan head moiety containing one or more sialic acids and a ceramide chain. The analysis of the glycan moieties among different subclass gangliosides, including GM, GD, and GT gangliosides, remains a challenge for shotgun lipidomics. Here, we present a novel shotgun lipidomics approach employing gas-phase ion/ion chemistry. The gas-phase derivatization strategy provides a rapid way to manipulate the ion-types of the precursor ions, and, in conjunction with collision induced dissociation (CID), allows for the elucidation of the structures of the glycan moieties from gangliosides. In addition to the enhancement of structural characterization, gas-phase ion chemistry leads to a form of purification

SUBMITTER: Chao HC 

PROVIDER: S-EPMC9648625 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature