Quantifying how post-transcriptional noise and gene copy number variation bias transcriptional parameter inference from mRNA distributions.

ABSTRACT: Transcriptional rates are often estimated by fitting the distribution of mature mRNA numbers measured using smFISH (single molecule fluorescence in situ hybridization) with the distribution predicted by the telegraph model of gene expression, which defines two promoter states of activity and inactivity. However, fluctuations in mature mRNA numbers are strongly affected by processes downstream of transcription. In addition, the telegraph model assumes one gene copy but in experiments, cells may have two gene copies as cells replicate their genome during the cell cycle. While it is often presumed that post-transcriptional noise and gene copy number variation affect transcriptional parameter estimation, the size of the error introduced remains unclear. To address this issue, here we me

SUBMITTER: Fu X 

PROVIDER: S-EPMC9648968 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature