Development of an Independent Prognostic Signature Based on Three Hypoxia-Related Genes for Breast Cancer

ABSTRACT:

Background

Hypoxia was considered to be a prognostic indicator in a variety of solid tumors. This study aims at identifying the hypoxia-related genes (HRGs) in breast cancer (BC) and the feasibility of HRGs as a prognostic indicator.

Methods

We downloaded the mRNA expression data of BC patients from TCGA and GEO databases. The LASSO Cox regression analysis was applied to screen the hub HRGs to establish a prognostic Risk Score. The independence of Risk Score was assessed by multivariate Cox regression analysis. And the immune checkpoint analysis was also performed. In addition, we also detected the expression level of hub HRGs in MCF-10A cells, MCF-7 cells, and SK-BR-3 cells by RT-qPCR.

Results

Three HRGs were identified as hub genes with prognostic value in B

SUBMITTER: Wang H 

PROVIDER: S-EPMC9649307 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature