Application of next-generation sequencing for the high-resolution typing of MHC-B in Korean native chicken.

ABSTRACT: The major histocompatibility complex-B (MHC-B) region of chicken is crucially important in their immunogenesis and highly diverse among different breeds, lines, and even populations. Because it determines the resistance/susceptibility to numerous infectious diseases, it is important to analyze this genomic region, particularly classical class I and II genes, to determine the variation and diversity that ultimately affect antigen presentation. This study investigated five lines of indigenous Korean native chicken (KNC) and the Ogye breed using next-generation sequencing (NGS) data with Geneious Prime-based assembly and variant calling with the Genome Analysis Toolkit (GATK) best practices pipeline. The consensus sequences of MHC-B (BG1-BF2) were obtained f

SUBMITTER: Ediriweera TK 

PROVIDER: S-EPMC9649677 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature