Transcription suppression is mediated by the HDAC1-Sin3 complex in Xenopus nucleoplasmic extract.

ABSTRACT: Modification of histones provides a dynamic mechanism to regulate chromatin structure and access to DNA. Histone acetylation, in particular, plays a prominent role in controlling the interaction between DNA, histones, and other chromatin-associated proteins. Defects in histone acetylation patterns interfere with normal gene expression and underlie a wide range of human diseases. Here, we utilize Xenopus egg extracts to investigate how changes in histone acetylation influence transcription of a defined gene construct. We show that inhibition of histone deacetylase 1 and 2 (HDAC1/2) specifically counteracts transcription suppression by preventing chromatin compaction and deacetylation of histone residues H4K5 and H4K8. Acetylation of these sites supports binding of the chromatin reader and t

SUBMITTER: Quaas CE 

PROVIDER: S-EPMC9650048 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature