Probing the sORF-Encoded Peptides of Deinococcus radiodurans in Response to Extreme Stress.

ABSTRACT: Organisms have developed different mechanisms to respond to stresses. However, the roles of small ORF-encoded peptides (SEPs) in these regulatory systems remain elusive, which is partially because of the lack of comprehensive knowledge regarding these biomolecules. We chose the extremophile Deinococcus radiodurans R1 as a model species and conducted large-scale profiling of the SEPs related to the stress response. The integrated workflow consisting of multiple omics approaches for SEP identification was streamlined, and an SEPome of D. radiodurans containing 109 novel and high-confidence SEPs was drafted. Forty-four percent of these SEPs were predicted to function as antimicrobial peptides. Quantitative peptidomics analysis indicated that the expression of SEP068184 was upregulated upon ox

SUBMITTER: Zhou C 

PROVIDER: S-EPMC9650054 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature