Astrocytes exhibit diverse Ca²⁺ changes at subcellular domains during brain aging.

ABSTRACT: Astrocytic Ca²⁺ transients are essential for astrocyte integration into neural circuits. These Ca²⁺ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in astrocytes. Until now, it was unclear whether and how aging affects astrocytic Ca²⁺ transients at subcellular domains. In this study, we combined a genetically encoded Ca²⁺ sensor (GCaMP6f) and in vivo two-photon Ca²⁺ imaging to determine changes in Ca²⁺ transients within astrocytic subcellular domains during brain aging. We showed that aging increased Ca²⁺ transients in astrocytic primary branches, higher-order

SUBMITTER: Ding F 

PROVIDER: S-EPMC9650392 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature