Dynamic regulation of Zn(II) sequestration by calgranulin C.

ABSTRACT: Calgranulin C performs antimicrobial activity in the human immune response by sequestering Zn(II). This biological function is afforded with the aid of two structurally distinct Ca(II)-binding EF hand motifs, wherein one of which bears an unusual amino acid sequence. Here, we utilize solution state NMR relaxation measurements to investigate the mechanism of Ca(II)-modulated enhancement of Zn(II) sequestration by calgranulin C. Using C¹³ /N¹⁵ CPMG dispersion experiments we have measured pH-dependent major and minor state populations exchanging on micro-to-millisecond timescale. This conformational exchange takes place exclusively in the Ca(II)-bound state and can be mapped to residues located in the EF-I loop and the linker between the tandem EF hands. Molecular dynami

SUBMITTER: Wang Q 

PROVIDER: S-EPMC9650546 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature