Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements.

ABSTRACT: Single-molecule imaging is invaluable for investigating the heterogeneous behavior and interactions of biological molecules. However, an impediment to precise sampling of single molecules is the irreversible adsorption of components onto the surfaces of cover glasses. This causes continuous changes in the concentrations of different molecules dissolved or suspended in the aqueous phase from the moment a sample is dispensed, which will shift, over time, the position of chemical equilibria between monomeric and multimeric components. Interferometric scattering microscopy (iSCAT) is a technique in the single-molecule toolkit that has the capability to detect unlabeled proteins and protein complexes both as they adsorb onto and desorb from a glass surface. Here, we examine the reversible and i

SUBMITTER: Bueno-Alejo CJ 

PROVIDER: S-EPMC9650645 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature