Minor histocompatibility antigens HA-8 and PANE1 in the TUNISIAN population.

ABSTRACT:

Background

Minor histocompatibility antigens (mHAgs) are endogenous immunogenic peptides initially identified due to complications detected in several contexts of HLA geno-identical hematopoietic stem cell transplantation (HSCT). In this study, we chose to examine the molecular polymorphism of the mHAgs HA-8 and PANE1 in the Tunisian population.

Material and methods

This study was conducted on 150 healthy and unrelated individuals. The DNA extraction and Sequence-Specific Primers PCR (PCR-SSP) methods were used for the molecular genotyping of the selected SNPs: PUM3 (rs2173904) and CENPM (rs5758511).

Results

Our results show that, 94% of Tunisians are carriers of the PANE1R allele (immunogenic variant of the PANE1 mHAg) and 68% of Tunisians are carriers of the HA-8R a

SUBMITTER: Said R 

PROVIDER: S-EPMC9651600 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature