MetaGT: A pipeline for de novo assembly of metatranscriptomes with the aid of metagenomic data.

ABSTRACT: While metagenome sequencing may provide insights on the genome sequences and composition of microbial communities, metatranscriptome analysis can be useful for studying the functional activity of a microbiome. RNA-Seq data provides the possibility to determine active genes in the community and how their expression levels depend on external conditions. Although the field of metatranscriptomics is relatively young, the number of projects related to metatranscriptome analysis increases every year and the scope of its applications expands. However, there are several problems that complicate metatranscriptome analysis: complexity of microbial communities, wide dynamic range of transcriptome expression and importantly, the lack of high-quality computational methods for assembling meta-RNA sequen

SUBMITTER: Shafranskaya D 

PROVIDER: S-EPMC9651917 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature