Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy.

ABSTRACT: Brain function is mediated by the physiological coordination of a vast, intricately connected network of molecular and cellular components. The physiological properties of neural network components can be quantified with high throughput. The ability to assess many animals per study has been critical in relating physiological properties to behavior. By contrast, the synaptic structure of neural circuits is presently quantifiable only with low throughput. This low throughput hampers efforts to understand how variations in network structure relate to variations in behavior. For neuroanatomical reconstruction, there is a methodological gulf between electron microscopic (EM) methods, which yield dense connectomes at considerable expense and low throughput, and light microscopic (LM) methods, wh

SUBMITTER: Lillvis JL 

PROVIDER: S-EPMC9651950 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature