Metformin Suppresses Hepatocellular Carcinoma through Regulating Alternative Splicing of LGR4.

ABSTRACT:

Methods

First, the expression of LGR4 in HCC tumor tissues and cell lines was detected by western blotting and immunofluorescence. The ability of cell proliferation, migration, and invasion was detected with CCK8, wound-healing, and transwell assays when overexpressing LGR4 or treating with metformin. The β-catenin expression was detected by immunofluorescence. In order to investigate novel AS-associated LGR4, we discarded LGR4 isoforms from GSO databases. We used siRNA to knock down the specific isoform to check the cell proliferation, migration, and invasion when treated with metformin.

Results

The level of LGR4 expression was higher in HCC cell lines and tumor tissues. The HCC cell proliferation, migration, and invasion were increased when overexpressing LGR4, whic

SUBMITTER: Zhuo H 

PROVIDER: S-EPMC9652085 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature