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Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo.


ABSTRACT: Here we describe the microglia-targeting adeno-associated viral (AAV) vectors containing a 1.7-kb putative promoter region of microglia/macrophage-specific ionized calcium-binding adaptor molecule 1 (Iba1), along with repeated miRNA target sites for microRNA (miR)-9 and miR-129-2-3p. The 1.7-kb genomic sequence upstream of the start codon in exon 1 of the Iba1 (Aif1) gene, functions as microglia preferential promoter in the striatum and cerebellum. Furthermore, ectopic transgene expression in non-microglial cells is markedly suppressed upon adding two sets of 4-repeated miRNA target sites for miR-9 and miR-129-2-3p, which are expressed exclusively in non-microglial cells and sponged AAV-derived mRNAs. Our vectors transduced ramified microglia in healthy tissues and reactive microglia in lipopolysaccharide-treated mice and a mouse model of neurodegenerative disease. Moreover, live fluorescent imaging allowed the monitoring of microglial motility and intracellular Ca2+ mobilization. Thus, microglia-targeting AAV vectors are valuable for studying microglial pathophysiology and therapies, particularly in the striatum and cerebellum.

SUBMITTER: Okada Y 

PROVIDER: S-EPMC9652230 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo.

Okada Yukihiro Y   Hosoi Nobutake N   Matsuzaki Yasunori Y   Fukai Yuuki Y   Hiraga Akito A   Nakai Junichi J   Nitta Keisuke K   Shinohara Yoichiro Y   Konno Ayumu A   Hirai Hirokazu H  

Communications biology 20221111 1


Here we describe the microglia-targeting adeno-associated viral (AAV) vectors containing a 1.7-kb putative promoter region of microglia/macrophage-specific ionized calcium-binding adaptor molecule 1 (Iba1), along with repeated miRNA target sites for microRNA (miR)-9 and miR-129-2-3p. The 1.7-kb genomic sequence upstream of the start codon in exon 1 of the Iba1 (Aif1) gene, functions as microglia preferential promoter in the striatum and cerebellum. Furthermore, ectopic transgene expression in no  ...[more]

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