Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells.

ABSTRACT: Cancer cells feature a resting membrane potential (V_m) that is depolarized compared to normal cells, and express active ionic conductances, which factor directly in their pathophysiological behavior. Despite similarities to 'excitable' tissues, relatively little is known about cancer cell V_m dynamics. Here high-throughput, cellular-resolution V_m imaging reveals that V_m fluctuates dynamically in several breast cancer cell lines compared to non-cancerous MCF-10A cells. We characterize V_m fluctuations of hundreds of human triple-negative breast cancer MDA-MB-231 cells. By quantifying their Dynamic Electrical Signatures (DESs) through an unsupervised machine-learning protocol, we identify four classes ranging from "noisy" to "blinking/wavi

SUBMITTER: Quicke P 

PROVIDER: S-EPMC9652252 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature