Amplification and sequencing of entire tick mitochondrial genomes for a phylogenomic analysis.

ABSTRACT: The mitochondrial genome (mitogenome) has proven to be important for the taxonomy, systematics, and population genetics of ticks. However, current methods to generate mitogenomes can be cost-prohibitive at scale. To address this issue, we developed a cost-effective approach to amplify and sequence the whole mitogenome of individual tick specimens. Using two different primer sites, this approach generated two full-length mitogenome amplicons that were sequenced using the Oxford Nanopore Technologies' Mk1B sequencer. We used this approach to generate 85 individual tick mitogenomes from samples comprised of the three tick families, 11 genera, and 57 species. Twenty-six of these species did not have a complete mitogenome available on GenBank prior to this work. We benchmarked the accuracy of t

SUBMITTER: Kneubehl AR 

PROVIDER: S-EPMC9652274 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature