The downregulation of LINC00273 inhibits the proliferation, invasion, and migration of ovarian cancer cells in vivo and in vitro.

ABSTRACT:

Background

This study sought to analyze long non-coding ribonucleic acid (lncRNA) LINC00273 expression in ovarian cancer tissues, and to preliminarily explore its effect on the growth and invasion of ovarian cancer cells and its influencing mechanism.

Methods

Quantitative real-time polymerase chain reaction was performed to detect the LINC00273 expression levels of cancerous ovarian tissues and their related cell lines. The ovarian cancer cells with the highest expression of LINC00273 were transfected with a knockdown lentiviral vector targeting the LINC00273 sequence and a negative control plasmid. The effects of the LINC00273 knockdown on the invasion and growth of these cancerous cells were evaluated by clonogenic assays, flow cytometry, EdU (5-Ethynyl-2'-deoxyuridine), C

SUBMITTER: Shu C 

PROVIDER: S-EPMC9652518 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature