YAP/TAZ Mediate TGFβ2-Induced Schlemm's Canal Cell Dysfunction.

ABSTRACT:

Purpose

Elevated transforming growth factor beta2 (TGFβ2) levels in the aqueous humor have been linked to glaucomatous outflow tissue dysfunction. Potential mediators of dysfunction are the transcriptional coactivators, Yes-associated protein (YAP) and transcriptional coactivator with PDZ binding motif (TAZ). However, the molecular underpinnings of YAP/TAZ modulation in Schlemm's canal (SC) cells under glaucomatous conditions are not well understood. Here, we investigate how TGFβ2 regulates YAP/TAZ activity in human SC (HSC) cells using biomimetic extracellular matrix hydrogels, and examine whether pharmacological YAP/TAZ inhibition would attenuate TGFβ2-induced HSC cell dysfunction.

Methods

Primary HSC cells were seeded atop photo-cross-linked extracellular matrix hydrogels

SUBMITTER: Li H 

PROVIDER: S-EPMC9652721 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature