Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material.

ABSTRACT:

Background

Recent technological advances opened the opportunity to simultaneously study gene expression for thousands of individual cells on a genome-wide scale. The experimental accessibility of such single-cell RNA sequencing (scRNAseq) approaches allowed gaining insights into the cell type composition of heterogeneous tissue samples of animal model systems and emerging models alike. A major prerequisite for a successful application of the method is the dissociation of complex tissues into individual cells, which often requires large amounts of input material and harsh mechanical, chemical and temperature conditions. However, the availability of tissue material may be limited for small animals, specific organs, certain developmental stages or if samples need to be acquired from c

SUBMITTER: Wiegleb G 

PROVIDER: S-EPMC9652833 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature