A CRISPR-based chromosomal-separation technique for Escherichia coli.

ABSTRACT:

Background

Natural life systems can be significantly modified at the genomic scale by human intervention, demonstrating the great innovation capacity of genome engineering. Large epi-chromosomal DNA structures were established in Escherichia coli cells, but some of these methods were inconvenient, using heterologous systems, or relied on engineered E. coli strains.

Results

The wild-type model bacterium E. coli has a single circular chromosome. In this work, a novel method was developed to split the original chromosome of wild-type E. coli. With this method, novel E. coli strains containing two chromosomes of 0.10 Mb and 4.54 Mb, and 2.28 Mb and 2.36 Mb were created respectively, designated as E. coli^0.10/4.54 and E. coli^2.28/2.36. The new chromosomal ar

SUBMITTER: Su J 

PROVIDER: S-EPMC9652834 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature