Photolysis of caged cytokinin in single cells of Arabidopsis thaliana.

ABSTRACT:

Background

Cytokinins are a class of phytohormone that play a crucial role in the development of plants. They are involved in the regulation of nearly every aspect of plant growth, from germination to senescence. The role of cytokinins in many developmental programs is complex and varies both spatially and temporally. Current techniques used to investigate the functions of cytokinins in plant development lack this spatial and temporal resolution required to observe cell-type specific effects.

Results

To this end, we present a method of activating a caged cytokinin in single cells. A caged benzyladenine was synthesized, along with caged adenine as a negative control. In vitro testing confirmed ultraviolet light-mediated uncaging, and subsequent root growth assays demonstrated

SUBMITTER: Dow L 

PROVIDER: S-EPMC9652950 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature