Intestinal gluconeogenesis is downregulated in pediatric patients with celiac disease.

ABSTRACT:

Background

Untreated celiac disease (CD) patients have increased levels of blood glutamine and a lower duodenal expression of glutaminase (GLS). Intestinal gluconeogenesis (IGN) is a process through which glutamine is turned into glucose in the small intestine, for which GLS is crucial. Animal studies suggest impaired IGN may have long-term effects on metabolic control and be associated with the development of type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). The aim of this study was to thoroughly investigate IGN at the gene expression level in children with untreated celiac disease.

Methods

Quantitative polymerase chain reaction (qPCR) was used to quantify the expression of 11 target genes related to IGN using the delta-delta Ct method with three reference gen

SUBMITTER: Karlson O 

PROVIDER: S-EPMC9652951 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature