Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods.

ABSTRACT: An essential basis for objectively improving the status of animals during in vivo research is the ability to measure the wellbeing of animals in a reliable and scientific manner. Several non-invasive methods such as assessing body weight, burrowing activity, nesting behavior, a distress score and fecal corticosterone metabolites were evaluated in healthy mice and after three surgical interventions or during the progression of four gastrointestinal diseases. The performance of each method in differentiating between healthy and diseased animals was assessed using receiver operating characteristic curves. The ability to differentiate between these two states differed between distinct surgical interventions and distinct gastrointestinal diseases. Thus, the generalizability of these methods for

SUBMITTER: Zechner D 

PROVIDER: S-EPMC9654551 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature