Association of Clinical Features with Spike Glycoprotein Mutations in Iranian COVID-19 Patients.

ABSTRACT:

Background

Mutations in spike glycoprotein, a critical protein of SARS-CoV-2, could directly impact pathogenicity and virulence. The D614G mutation, a non-synonymous mutation at position 614 of the spike glycoprotein, is a predominant variant circulating worldwide. This study investigated the occurrence of mutations in the crucial zone of the spike gene and the association of clinical symptoms with spike mutations in isolated viruses from Iranian patients infected with SARS-CoV-2 during the second and third waves of the COVID-19 epidemic in Isfahan, the third-largest city in Iran.

Methods

The extracted RNA from 60 nasopharyngeal samples of COVID-19 patients were subjected to cDNA synthesis and RT-PCR (in three overlapping fragments). Each patient's reverse transcriptase poly

SUBMITTER: Ahangarzadeh S 

PROVIDER: S-EPMC9654899 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature