Brain Regional Identity and Cell Type Specificity Landscape of Human Cortical Organoid Models.

ABSTRACT: In vitro models of corticogenesis from pluripotent stem cells (PSCs) have greatly improved our understanding of human brain development and disease. Among these, 3D cortical organoid systems are able to recapitulate some aspects of in vivo cytoarchitecture of the developing cortex. Here, we tested three cortical organoid protocols for brain regional identity, cell type specificity and neuronal maturation. Overall, all protocols gave rise to organoids that displayed a time-dependent expression of neuronal maturation genes such as those involved in the establishment of synapses and neuronal function. Comparatively, guided differentiation methods without WNT activation generated the highest degree of cortical regional identity, whereas default conditions produced the broadest range of cell ty

SUBMITTER: Magni M 

PROVIDER: S-EPMC9654943 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature