Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in Aconitum gymnandrum Maxim.

ABSTRACT: Diterpene alkaloids (DAs) are characteristic compounds in Aconitum, which are classified into four skeletal types: C₁₈, C₁₉, C₂₀, and bisditerpenoid alkaloids. C₂₀-DAs are thought to be the precursor of the other types. Their biosynthetic pathway, however, is largely unclear. Herein, we combine metabolomics and transcriptomics to unveil the methyl jasmonate (MJ) inducible biosynthesis of DAs in the sterile seedling of A. gymnandrum, the only species in the Subgenus Gymnaconitum (Stapf) Rapaics. Target metabolomics based on root and aerial portions identified 51 C₁₉-DAs and 15 C₂₀-DAs, with 40 inducible compounds. The highest content of C₂₀-DA atisine was selected for further network analysis.

SUBMITTER: Chen L 

PROVIDER: S-EPMC9655601 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature