Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases.

ABSTRACT:

Background

Plant cell walls represent the most plentiful renewable organic resource on earth, but due to their heterogeneity, complex structure and partial recalcitrance, their use as biotechnological feedstock is still limited.

Results

In order to identify efficient enzymes for polysaccharide breakdown, we have carried out functional screening of metagenomic fosmid libraries from biogas fermenter microbial communities grown on sugar beet pulp, an arabinan-rich agricultural residue, or other sources containing microbes that efficiently depolymerize polysaccharides, using CPH (chromogenic polysaccharide hydrogel) or ICB (insoluble chromogenic biomass) labeled polysaccharide substrates. Seventy-one depolymerase-encoding genes were identified from 55 active fosmid clones by usi

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9655821 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature