A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application.

ABSTRACT: In this study, a potential producer of prebiotics, a novel endo-polygalacturonase pePGA from Penicillium rolfsii BM-6, was successfully expressed in Komagataella phaffii, characterized and applied to produce pectic oligosaccharides. The optimum temperature and pH of pePGA were 60 °C and 6.0. The purified recombinant enzyme showed a good pH stability and was stable from pH 3.5 to 8.0. The K_m, V_max and k_cat values of pePGA were 0.1569 g/L, 12,273 μmol/min/mg and 7478.4 s^-1, respectively. More importantly, pePGA-POS, the pePGA hydrolysis products from commercial pectin, had good prebiotic and antibacterial activities in vitro. The pePGA-POS was able to significantly promote the growth of probiotics; meanwhile, the

SUBMITTER: Hao MJ 

PROVIDER: S-EPMC9656037 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature