A Novel Plasma-Based Methylation Panel for Upper Gastrointestinal Cancer Early Detection.

ABSTRACT:

Background

Upper gastrointestinal cancer (UGC) is an important cause of cancer death in China, with low five-year survival rates due to the majority of UGC patients being diagnosed at an advanced stage. Therefore, there is an urgent need to develop cost-effective, reliable and non-invasive methods for the early detection of UGC.

Methods

A novel plasma-based methylation panel combining simultaneous detection of three methylated biomarkers (ELMO1, ZNF582 and TFPI2) and an internal control gene were developed and used to examine plasma samples from 186 UGC patients and 190 control subjects.

Results

The results indicated excellent PCR amplification efficiency and reproducibility of ELMO1, ZNF582 and TFPI2 in the range of 10-100,00

SUBMITTER: Peng C 

PROVIDER: S-EPMC9656240 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature