In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells.

ABSTRACT: Low back pain is a clinically highly relevant musculoskeletal burden and is associated with inflammatory as well as degenerative processes of the intervertebral disc. However, the pathophysiology and cellular pathways contributing to this devastating condition are still poorly understood. Based on previous evidence, we hypothesize that tissue renin-angiotensin system (tRAS) components, including the SARS-CoV-2 entry receptor angiotensin-converting enzyme 2 (ACE2), are present in human nucleus pulposus (NP) cells and associated with inflammatory and degenerative processes. Experiments were performed with NP cells from four human donors. The existence of angiotensin II, angiotensin II type 1 receptor (AGTR1), AGTR2, MAS-receptor (MasR), and ACE2 in human NP cells was validated with immunoflu

SUBMITTER: Saravi B 

PROVIDER: S-EPMC9656476 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature