Susceptibility to epilepsy after traumatic brain injury is associated with preexistent gut microbiome profile.

ABSTRACT:

Objective

We examined whether posttraumatic epilepsy (PTE) is associated with measurable perturbations in gut microbiome.

Methods

Adult Sprague Dawley rats were subjected to lateral fluid percussion injury (LFPI). PTE was examined 7 months after LFPI, during 4-week continuous video-electroencephalographic monitoring. 16S ribosomal RNA gene sequencing was performed in fecal samples collected before LFPI/sham-LFPI and 1 week, 1 month, and 7 months thereafter. Longitudinal analyses of alpha diversity, beta diversity, and differential microbial abundance were performed. Short-chain fatty acids (SCFAs) were measured in fecal samples collected before LFPI by liquid chromatography with tandem mass spectrometry.

Results

Alpha diversity changed over time in both LFPI and sham-

SUBMITTER: Medel-Matus JS 

PROVIDER: S-EPMC9656503 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature