High Frequency of Antibiotic Resistance Genes (ARGs) in the Lerma River Basin, Mexico.

ABSTRACT: The spread of beta-lactamase-producing bacteria is of great concern and the environment has been found to be a main source of contamination. Herein, it was proposed to determine the frequency of antimicrobial-resistant-Gram-negative bacteria throughout the Lerma River basin using phenotypic and molecular methods. Resistant bacteria were isolated with chromogenic media and antimicrobial susceptibility tests were used to characterize their resistance. ARGs for beta-lactams, aminoglycosides, and quinolones were detected by PCR. Species were identified by Sanger sequencing the 16S rRNA gene and the representative genomes of MDR strains were sequenced by NGS. A high variation in the number of isolates was observed in the 20 sampled sites, while observing a low diversity among the resistant bact

SUBMITTER: Tapia-Arreola AK 

PROVIDER: S-EPMC9657182 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature