Preparation of Temperature-Responsive Antibody-Nanoparticles by RAFT-Mediated Grafting from Polymerization.

ABSTRACT: Herein, we report the preparation of temperature-responsive antibody-nanoparticles by the direct polymerization of N-isopropylacrylamide (NIPAAm) from immunoglobulin G (IgG). To this end, a chain transfer agent (CTA) was introduced into IgG, followed by the precipitation polymerization of NIPAAm in an aqueous medium via reversible addition-fragmentation chain transfer polymerization above the lower critical solution temperature (LCST). Consequently, antibody-polymer particles with diameters of approximately 100-200 nm were formed. Owing to the entanglement of the grafted polymers via partial chemical crosslinking, the antibody-nanoparticles maintained their stability even at temperatures below the LCST. Further, the dispersed nanoparticles could be collected by thermal precipitation

SUBMITTER: Yoshihara E 

PROVIDER: S-EPMC9657504 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature