The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches.

ABSTRACT:

Background

Cytotoxicity testing is a primary method to establish the safety of biomaterials, e.g., biocomposites. Biomaterials involve a wide range of medical materials, which are usually solid materials and are used in bone regeneration, cardiology, or dermatology. Current advancements in science and technology provide several standard cytotoxicity testing methods that are sufficiently sensitive to detect various levels of cellular toxicity, i.e., from low to high. The aim was to compare the direct and indirect methodology described in the ISO guidelines UNE-EN ISO 10993-5:2009 Part 5.

Methods

Cell proliferation was measured using WST-1 assay, and cytotoxicity was measured using LDH test kit.

Results

The results indicate that the molecular surface of biomaterials hav

SUBMITTER: Chraniuk M 

PROVIDER: S-EPMC9657594 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature