Melatonin Attenuates H₂O₂-Induced Oxidative Injury by Upregulating LncRNA NEAT1 in HT22 Hippocampal Cells.

ABSTRACT: More research is required to understand how melatonin protects neurons. The study aimed to find out if and how long non-coding RNA (lncRNA) contributes to melatonin's ability to defend the hippocampus from H₂O₂-induced oxidative injury. LncRNAs related to oxidative injury were predicted by bioinformatics methods. Mouse hippocampus-derived neuronal HT22 cells were treated with H₂O₂ with or without melatonin. Viability and apoptosis were detected by Cell Counting Kit-8 and Hoechst33258. RNA and protein levels were measured by quantitative real-time PCR, Western blot, and immunofluorescence. Bioinformatics predicted that 38 lncRNAs were associated with oxidative injury in mouse neurons. LncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) was re

SUBMITTER: Gao Q 

PROVIDER: S-EPMC9657978 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature