Human Mesenchymal Stem Cell Secretome Driven T Cell Immunomodulation Is IL-10 Dependent.

ABSTRACT: The Human Mesenchymal Stem Cell (hMSC) secretome has pleiotropic effects underpinning its therapeutic potential. hMSC serum-free conditioned media (SFCM) contains a variety of cytokines, with previous studies linking a changed secretome composition to physoxia. The Jurkat T cell model allowed the efficacy of SFCM vs. serum-free media (SFM) in the suppression of immunological aspects, including proliferation and polarisation, to be explored. Cell growth in SFM was higher [(21% O₂ = 5.3 × 10⁵ ± 1.8 × 10⁴ cells/mL) and (2% O₂ = 5.1 × 10⁵ ± 3.0 × 10⁴ cells/mL)], compared to SFCM [(21% O₂ = 2.4 × 10⁵ ± 2.5 × 10⁴ cells/mL) and (2% O₂ = 2.2 × 10⁵ ± 5.8 × 10³ cells/mL

SUBMITTER: Shephard MT 

PROVIDER: S-EPMC9658100 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature