Nano-positioning and tubulin conformation contribute to axonal transport regulation of mitochondria along microtubules.

ABSTRACT: Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning and is regulated by intracellular transport mechanisms. Controlled transport of bulky mitochondria is especially important in polarized cells such as neurons that rely on these organelles to locally produce energy and buffer calcium. Mitochondrial transport requires and depends on microtubules that fill much of the available axonal space. How mitochondrial transport is affected by their position within the microtubule bundles is not known. Here, we found that anterograde transport, driven by kinesin motors, is susceptible to the molecular conformation of tubulin in neurons both in vitro and in vivo. Anterograde velocities negatively correlate with the density of elongated tubulin dimers l

SUBMITTER: Van Steenbergen V 

PROVIDER: S-EPMC9659393 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature