Severe choline deficiency induces alternative splicing aberrance in optimized duck primary hepatocyte cultures.

ABSTRACT:

Objective

Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model.

Methods

We performed an optimization culture meth

SUBMITTER: Zhao L 

PROVIDER: S-EPMC9659469 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature