Evaluation of candidate reference genes stability for gene expression analysis by reverse transcription qPCR in Clostridium perfringens.

ABSTRACT: Identification of stable reference genes for normalization purposes is necessary for obtaining reliable and accurate results of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyses. To our knowledge, no reference gene(s) have been validated for this purpose in Clostridium perfringens. In this study, the expression profile of ten candidate reference genes from three strains of C. perfringens were assessed for stability under various experimental conditions using geNorm in qbase + . These stability rankings were then compared to stability assessments evaluated by BestKeeper, NormFinder, delta Ct, and RefFinder algorithms. When comparing all the analyses; gyrA, ftsZ, and recA were identified within the most stable genes under the different experimental conditions an

SUBMITTER: Williams ML 

PROVIDER: S-EPMC9659559 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature