Biochemical and functional characterization of Brucella abortus cyclophilins: So similar, yet so different.

ABSTRACT: Brucella spp. are the etiological agent of animal and human brucellosis. We have reported previously that cyclophilins of Brucella (CypA and CypB) are upregulated within the intraphagosomal replicative niche and required for stress adaptation and host intracellular survival and virulence. Here, we characterize B. abortus cyclophilins, CypA, and CypB from a biochemical standpoint by studying their PPIase activity, chaperone activity, and oligomer formation. Even though CypA and CypB are very similar in sequence and share identical chaperone and PPIase activities, we were able to identify outstanding differential features between them. A series of differential peptide loops were predicted when comparing CypA and CypB, differences that might explain why specific antibodie

SUBMITTER: Muruaga EJ 

PROVIDER: S-EPMC9659587 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature