Interactions in CSF1-Driven Tenosynovial Giant Cell Tumors.

ABSTRACT:

Purpose

A major component of cells in tenosynovial giant cell tumor (TGCT) consists of bystander macrophages responding to CSF1 that is overproduced by a small number of neoplastic cells with a chromosomal translocation involving the CSF1 gene. An autocrine loop was postulated where the neoplastic cells would be stimulated through CSF1R expressed on their surface. Here, we use single-cell RNA sequencing (scRNA-seq) to investigate cellular interactions in TGCT.

Experimental design

A total of 18,788 single cells from three TGCT and two giant cell tumor of bone (GCTB) samples underwent scRNA-seq. The three TGCTs were additionally analyzed using long-read RNA sequencing. Immunofluorescence and IHC for a range of markers were used to validate and extend the scRNA-seq findings.

SUBMITTER: van IJzendoorn DGP 

PROVIDER: S-EPMC9660542 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature