Age dependent contribution of entry via the CSF to the overall brain entry of small and large hydrophilic markers.

ABSTRACT:

Background

Apparent permeability of the blood brain barrier to hydrophilic markers has been shown to be higher in the developing brain. Apart from synthesis in situ, any substance detected in the brain parenchyma can originate from two sources: directly through blood vessels of brain vasculature and/or indirectly by entry from the cerebrospinal fluid (CSF) after transfer across the choroid plexuses. The relative quantitative contribution of these two routes to the overall brain entry remains unclear.

Methods

In rats at embryonic day 16, 19 and postnatal day 4 and young adults, a small (sucrose, mw. 342 Da) or a large (dextran, mw. 70 kDa) radiolabelled hydrophilic marker was injected intravenously for very short periods of time (30 s to 5 min) before collection of plasma, ce

SUBMITTER: Qiu F 

PROVIDER: S-EPMC9661750 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature