AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination.

ABSTRACT:

Background

Cancer stem cells (CSCs) are regarded as the "seed cells" for tumorigenesis, metastasis, recurrence and drug resistance. However, specific surface markers of CSCs of different origins have not been documented.

Methods

Single-cell sequencing was used to analyze the highly expressed genes in cancer stem cells of gastric cancer patients, and it was verified that AQP5 was specifically highly expressed in gastric cancer stem cells (GC-CSCs) in vivo and in vitro. The effect of AQP5-promoting LGR5 on the malignant biological function of GC-CSCs was investigated. The mechanism by which AQP5 affects GC-CSCs was explored through transcriptome sequencing, proteomic detection, mass spectrometry, etc. RESULTS: We report the identification and validation of AQP5 as a potentiall

SUBMITTER: Zhao R 

PROVIDER: S-EPMC9661769 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature