Dual transcriptome of Streptococcus mutans and Candida albicans interplay in biofilms.

ABSTRACT:

Objective

To assess the interactions between Streptococcus mutans and Candida albicans during cariogenic biofilm formation.

Methods

The S. mutans and C. albicans duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy. The transcriptome of 48h-biofilms was assessed by RNA-Seq. The expression of S. mutans and C. albicans virulence genes was examined via real-time reverse transcription-polymerase chain reaction.

Results

The morphogenesis of C. albicans-S. mutans duo-species biofilms was significantly altered when comparing to S. mutans or C. albicans single-species biofilm. Duo-species biofilms exhi

SUBMITTER: Xiao J 

PROVIDER: S-EPMC9662060 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature