Targeted Metagenomic Sequencing-based Approach Applied to 2146 Tissue and Body Fluid Samples in Routine Clinical Practice.

ABSTRACT:

Background

The yield of next-generation sequencing (NGS) added to a Sanger sequencing-based 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) assay was evaluated in clinical practice for diagnosis of bacterial infection.

Methods

PCR targeting the V1 to V3 regions of the 16S rRNA gene was performed, with amplified DNA submitted to Sanger sequencing and/or NGS (Illumina MiSeq) or reported as negative, depending on the cycle threshold value. A total of 2146 normally sterile tissues or body fluids were tested between August 2020 and March 2021. Clinical sensitivity was assessed in 579 patients from whom clinical data were available.

Results

Compared with Sanger sequencing alone (400 positive tests), positivity increased by 87% by adding NGS (347 added positive

SUBMITTER: Flurin L 

PROVIDER: S-EPMC9662179 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature