Regulated degradation of the inner nuclear membrane protein SUN2 maintains nuclear envelope architecture and function.

ABSTRACT: Nuclear architecture and functions depend on dynamic interactions between nuclear components (such as chromatin) and inner nuclear membrane (INM) proteins. Mutations in INM proteins interfering with these interactions result in disease. However, mechanisms controlling the levels and turnover of INM proteins remain unknown. Here, we describe a mechanism of regulated degradation of the INM SUN domain-containing protein 2 (SUN2). We show that Casein Kinase 2 and the C-terminal domain Nuclear Envelope Phosphatase 1 (CTDNEP1) have opposing effects on SUN2 levels by regulating SUN2 binding to the ubiquitin ligase Skp/Cullin1/F-Box^βTrCP (SCF^βTrCP). Upon binding to phosphorylated SUN2, SCF^βTrCP promotes its ubiquitination. Ubiquitinated SUN2 is membrane extracted b

SUBMITTER: Krshnan L 

PROVIDER: S-EPMC9662817 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature