Comparison of next-generation sequencing and cobas EGFR mutation test v2 in detecting EGFR mutations.

ABSTRACT:

Background

As the number of genetic mutations that must be tested increases, the Oncomine Dx Target test (ODxTT), which can simultaneously detect multiple cancer-related genes is becoming the main test used in preference to single-molecule testing. In this study, we evaluated the performance of ODxTT and cobas EGFR mutation test v2 (cobas EGFR), one of the single-molecule tests, in detecting EGFR mutations.

Methods

Samples from 211 patients diagnosed with NS-NSCLC were tested simultaneously or sequentially with the cobas EGFR mutation test and ODxTT. We compared the success and detection rates of both tests and evaluated their equivalence by determining the concordance rate and k-coefficient of both tests.

Results

The success rate in detecting EGFR mutations was 95.7%

SUBMITTER: Murakami S 

PROVIDER: S-EPMC9663664 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature